abstract：:A whole-cell bacterial biosensor for measuring aqueous concentrations of aromatic aldehydes was developed. It is based on the E. coli BL21DE3(RIL) expressing the green fluorescent protein under the control of an alcohol dehydrogenase inducible promoter belonging to the archaeon Sulfolobus solfataricus (Sso2536adh prom...

journal_title：Applied microbiology and biotechnology

authors： Fiorentino G,Ronca R,Bartolucci S

更新日期：2009-02-01 00:00:00

abstract：:Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed m...

journal_title：Applied microbiology and biotechnology

authors： Temudo MF,Mato T,Kleerebezem R,van Loosdrecht MC

更新日期：2009-02-01 00:00:00

abstract：:The objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. We identified and selected specific genes to detect Yersinia pestis, Francisella tularensis, Burkholderia mallei, Burkhol...

journal_title：Applied microbiology and biotechnology

authors： Carrera M,Sagripanti JL

更新日期：2009-01-01 00:00:00

abstract：:A novel microbial transglutaminase (TGase) from the cultural filtrate of Streptomyces netropsis BCRC 12429 (Sn) was purified. The specific activity of the purified TGase was 18.2 U/mg protein with an estimated molecular mass of 38 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The TGase gen...

journal_title：Applied microbiology and biotechnology

authors： Yu YJ,Wu SC,Chan HH,Chen YC,Chen ZY,Yang MT

更新日期：2008-12-01 00:00:00

abstract：:The Baeyer-Villiger monooxygenase (BVMO) BmoF1 from Pseudomonas fluorescens DSM 50106 was shown before to enantioselectively oxidize different 4-hydroxy-2-ketones to the corresponding hydroxyalkyl acetates, being the first example of a BVMO-catalyzed kinetic resolution of aliphatic acyclic ketones. However, the wild-t...

journal_title：Applied microbiology and biotechnology

authors： Kirschner A,Bornscheuer UT

更新日期：2008-12-01 00:00:00

abstract：:One solution to the global crisis of antibiotic resistance is the discovery of novel antimicrobial compounds for clinical application. Marine organisms are an attractive and, as yet, relatively untapped resource of new natural products. Cell extracts from the marine diatom, Phaeodactylum tricornutum, have antibacteria...

journal_title：Applied microbiology and biotechnology

authors： Desbois AP,Lebl T,Yan L,Smith VJ

更新日期：2008-12-01 00:00:00

abstract：:At present, more than 100 strains of Lentinula edodes are cultivated on a commercial scale in China. A simple, reliable, and effective method to distinguish some commercial strains of the superior type from other commercial strains is very important for the Lentinula industry. In this study, 23 commercial strains of L...

journal_title：Applied microbiology and biotechnology

authors： Li HB,Wu XQ,Peng HZ,Fu LZ,Wei HL,Wu QQ,Jin QY,Li N

更新日期：2008-11-01 00:00:00

abstract：:Chlorogenate hydrolase (EC 3.1.1.42, CHase) was highly induced in mycelia of Aspergillus sojae AKU 3312 grown in Czapek medium containing either instant coffee powder or coffee pulp as inducer. No CHase formation was observed in the mycelia when cultivated without the inducer. CHase was purified readily from CHase-ind...

journal_title：Applied microbiology and biotechnology

authors： Adachi O,Ano Y,Akakabe Y,Shinagawa E,Matsushita K

更新日期：2008-11-01 00:00:00

abstract：:Immunostimulatory glucose polymers known as beta-glucans have been studied for many years. Our laboratory has prepared and characterized a novel microparticulate beta-glucan (MG) from the budding yeast Saccharomyces cerevisiae. Because MG particles are rapidly phagocytized by murine peritoneal macrophages and induce t...

journal_title：Applied microbiology and biotechnology

authors： Berner VK,Sura ME,Hunter KW Jr

更新日期：2008-10-01 00:00:00

abstract：:The construction of a new phagemid vector for display of peptides on the pVIII major coat protein of filamentous bacteriophage is described, in which expression of pVIII-peptide fusions was placed under the control of the arabinose-inducible P(BAD) promoter. The new phagemid showed excellent capacity for the regulatio...

journal_title：Applied microbiology and biotechnology

authors： Fagerlund A,Myrset AH,Kulseth MA

更新日期：2008-10-01 00:00:00

abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

journal_title：Applied microbiology and biotechnology

authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

更新日期：2008-10-01 00:00:00

abstract：:Comparative metabolic responses of Sinorhizobium sp. C4 were investigated. Comprehensive metabolites profiles, including polar metabolites, fatty acids, and polyhydroxyalkanoates were evaluated through untargeted metabolome analyses. Intracellular metabolomes during the degradation of phenanthrene were compared with t...

journal_title：Applied microbiology and biotechnology

authors： Keum YS,Seo JS,Li QX,Kim JH

更新日期：2008-10-01 00:00:00

abstract：:The objectives of this work were to demonstrate the potential of a two-phase sequencing batch reactor in degrading xenobiotics and to evaluate the kinetic parameters leading to a mathematical model of the system. 4-Nitrophenol (4NP), a typical representative of substituted phenols, was selected as the target xenobioti...

journal_title：Applied microbiology and biotechnology

authors： Tomei MC,Annesini MC,Rita S,Daugulis AJ

更新日期：2008-10-01 00:00:00

abstract：:The gene encoding NADP(+)-dependent L-1-amino-2-propanol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 was cloned and sequenced. A 780-bp nucleotide fragment was confirmed to be the gene encoding AADH by agreement of the N-terminal and internal amino acid sequences of the purified AADH. The gene (aadh) codes...

journal_title：Applied microbiology and biotechnology

authors： Kataoka M,Ishige T,Urano N,Nakamura Y,Sakuradani E,Fukui S,Kita S,Sakamoto K,Shimizu S

更新日期：2008-09-01 00:00:00

abstract：:Shewanella decolorationis S12, a representative dissimilatory azo-reducing bacterium of Shewanella genus, can grow by coupling the oxidation of hydrogen to the reduction of azo compounds as the sole electron acceptor, indicating that an uptake hydrogenase is an important component for electron transfer for azoreductio...

journal_title：Applied microbiology and biotechnology

authors： Hong YG,Guo J,Sun GP

更新日期：2008-09-01 00:00:00

abstract：:A yeast protein fragment complementation assay (PCA) system based on dihydrofolate reductase (DHFR) is difficult to be operated because it is not as sensitive to trimethoprim (TMP) as the system using a prokaryotic microorganism. Here, the PCA system using DHFR, specific inhibitors, and a substrate in the yeast Saccha...

journal_title：Applied microbiology and biotechnology

authors： Shibasaki S,Sakata K,Ishii J,Kondo A,Ueda M

更新日期：2008-09-01 00:00:00

abstract：:The extremely thermophilic anaerobic archaeon strain, HJ21, was isolated from a deep-sea hydrothermal vent, could produce hyperthermophilic alpha-amylase, and later was identified as Thermococcus from morphological, biochemical, and physiological characteristics and the 16S ribosomal RNA gene sequence. The extracellul...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Lu Z,Lu M,Qin S,Liu H,Deng X,Lin Q,Chen J

更新日期：2008-09-01 00:00:00

abstract：:Bacillus thuringiensis is well known as an effective bio-insecticidal bacterium. However, the roles of B. thuringiensis to control plant diseases are not paid great attention to. In recent years, many new functions in protecting plants from pathogen infection have been discovered. For example, acyl homoserine lactone ...

journal_title：Applied microbiology and biotechnology

authors： Zhou Y,Choi YL,Sun M,Yu Z

更新日期：2008-09-01 00:00:00

abstract：:A medium-scale (0.77 l) air-cathode, brush-anode microbial fuel cell (MFC) operated in fed-batch mode using xylose (20 mM) generated a maximum power density of 13 +/- 1 W/m(3) (673 +/- 43 mW/m(2)). Xylose was rapidly removed (83.5%) within 8 h of a 60-h cycle, with 42.1% of electrons in intermediates (8.5 +/- 0.2 mM a...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Logan BE

更新日期：2008-09-01 00:00:00

abstract：:A transcriptional activator for actinorhodin biosynthesis, AtrA, was previously characterized in Streptomyces coelicolor A3(2), and an orthologue of atrA, named aveI, is identified in the Streptomyces avermitilis NRRL8165 genome (Uguru et al., Mol Microbiol, 58:131-150, 2005). In this study, genetic and functional cha...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lu Y,Chen J,Zhang W,Shu D,Qin Z,Yang S,Jiang W

更新日期：2008-08-01 00:00:00

abstract：:A new highly scalable microbial fuel cell (MFC) design, consisting of a series of cassette electrodes (CE), was examined for increasing power production from organic matter in wastewater. Each CE chamber was composed of a box-shaped flat cathode (two air cathodes on both sides) sandwiched in between two proton-exchang...

journal_title：Applied microbiology and biotechnology

authors： Shimoyama T,Komukai S,Yamazawa A,Ueno Y,Logan BE,Watanabe K

更新日期：2008-08-01 00:00:00

abstract：:The biofilm formation of Pseudomonas aeruginosa, an opportunistic human pathogen, is developed by cell-to-cell signaling, so-called quorum sensing (QS). To control the biofilm formation, we designed and synthesized new QS inhibitors of P. aeruginosa based on the structure of the previously known QS inhibitor, furanone...

journal_title：Applied microbiology and biotechnology

authors： Kim C,Kim J,Park HY,Park HJ,Lee JH,Kim CK,Yoon J

更新日期：2008-08-01 00:00:00

abstract：:Environmentally friendly control measures are needed for suppression of soilborne pathogens of vegetable crops in the Republic of Korea. In vitro challenge assays were used to screen approximately 500 bacterial isolates from 20 Korean greenhouse soils for inhibition of diverse plant pathogens. One isolate, Bacillus su...

journal_title：Applied microbiology and biotechnology

authors： Chung S,Kong H,Buyer JS,Lakshman DK,Lydon J,Kim SD,Roberts DP

更新日期：2008-08-01 00:00:00

abstract：:A cytoplasmic NADH oxidase (NOX) was purified from a soil bacteria, Brevibacterium sp. KU1309, which is able to grow in the medium containing 2-phenylethanol as the sole source of carbon under an aerobic condition. The enzyme catalyzed the oxidation of NADH to NAD+ involving two-electron reduction of O2 to H2O2. The m...

journal_title：Applied microbiology and biotechnology

authors： Hirano J,Miyamoto K,Ohta H

更新日期：2008-08-01 00:00:00

abstract：:The causes of Bacillus spore resistance remain unclear. Many structures including a highly compact envelope, low hydration of the protoplast, high concentrations of Ca-chelated dipicolinic acid, and the presence of small acid-soluble spore proteins seem to contribute to resistance. To evaluate the role of internal pro...

journal_title：Applied microbiology and biotechnology

authors： Nguyen Thi Minh H,Guyot S,Perrier-Cornet JM,Gervais P

更新日期：2008-08-01 00:00:00

abstract：:A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

journal_title：Applied microbiology and biotechnology

authors： Weignerová L,Filipi T,Manglová D,Kren V

更新日期：2008-07-01 00:00:00

abstract：:Magnetotactic bacteria are difficult to grow under defined conditions in culture, which has presented a major obstacle to commercial application of magnetosomes. We studied the relationships among the cell growth, magnetosome formation, dissolved oxygen concentration (DO), and the ability to supply oxygen to the cells...

journal_title：Applied microbiology and biotechnology

authors： Sun JB,Zhao F,Tang T,Jiang W,Tian JS,Li Y,Li JL

更新日期：2008-06-01 00:00:00

abstract：:Chitin was purified from Penaeus monodon and Crangon crangon shells using a two-stage fermentation process with anaerobic deproteination followed by decalcification through homofermentative lactic acid fermentation. Deproteinating enrichment cultures from sewage sludge and ground meat (GM) were used with a proteolytic...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Gallert C,Winter J

更新日期：2008-06-01 00:00:00

abstract：:A biosynthetic pathway for the production of (S)-3-hydroxybutyric acid (S3HB) from glucose was established in recombinant Escherichia coli by introducing the beta-ketothiolase gene from Ralstonia eutropha H16, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from R. eutropha H16, or Clostridium acetobutylicum ATCC824, ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Park SJ,Lee SY,Hong SH

更新日期：2008-06-01 00:00:00

abstract：:This study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medi...

journal_title：Applied microbiology and biotechnology

authors： Song H,Kim TY,Choi BK,Choi SJ,Nielsen LK,Chang HN,Lee SY

更新日期：2008-05-01 00:00:00

abstract：:Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to...

journal_title：Applied microbiology and biotechnology

authors： Barrios-González J,Baños JG,Covarrubias AA,Garay-Arroyo A

更新日期：2008-05-01 00:00:00

abstract：:Epoxide hydrolases (EHs) of fungal origin have the ability to catalyze the enantioselective hydrolysis of epoxides to their corresponding diols. However, wild type fungal EHs are limited in substrate range and enantioselectivity. Additionally, the production of fungal epoxide hydrolase (EH) by wild-type strains is typ...

journal_title：Applied microbiology and biotechnology

authors： Maharajh D,Roth R,Lalloo R,Simpson C,Mitra R,Görgens J,Ramchuran S

更新日期：2008-05-01 00:00:00

abstract：:Bioconversion of cassava-derived glucose to 2-keto-D-gluconic acid (2-KDG) using resting cells of immobilized Pseudomonas aeruginosa IFO 3448 was investigated. The tuberous roots of cassava were selected as the feedstock as they are inexpensive and widely available, and possess high amounts of starch (approximately 70...

journal_title：Applied microbiology and biotechnology

authors： Chia M,Van Nguyen TB,Choi WJ

更新日期：2008-04-01 00:00:00

abstract：:Microbial genome sequencing has left a legacy of annotated yet uncharacterized genes or open reading frames, activities that may have useful applications in health and/or the environment. We are interested in the discovery and characterization of potentially new pectinolytic activities for the enzymatic retting of nat...

journal_title：Applied microbiology and biotechnology

authors： Xiao Z,Boyd J,Grosse S,Beauchemin M,Coupe E,Lau PC

更新日期：2008-04-01 00:00:00

abstract：:Proteins that catalyze H2-pathways often contain iron-sulfur (Fe-S) clusters and are sensitive to O2. We tested whether deletion of the gene encoding the transcriptional negative regulator, IscR, could enhance the ability of Escherichia coli BL21 to synthesize active recombinant H2-pathway components and stimulate fer...

journal_title：Applied microbiology and biotechnology

authors： Akhtar MK,Jones PR

更新日期：2008-04-01 00:00:00

abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

journal_title：Applied microbiology and biotechnology

authors： Adav SS,Lee DJ

更新日期：2008-03-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechoc...

journal_title：Applied microbiology and biotechnology

authors： Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

更新日期：2008-03-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:Although host proteases are often considered to have a negative impact upon heterologous protein production by filamentous fungi, relatively little is known about the pattern of their appearance in recombinant fungal bioprocesses. In the present study, we investigated extracellular proteases from a filamentous fungus,...

journal_title：Applied microbiology and biotechnology

authors： Li Q,Harvey LM,McNeil B

更新日期：2008-02-01 00:00:00